Diversification of the Amazon biota: Rivers as vicariant barriers.

-Download georeferenced distributions through the Global Biodiversity Information Facility (GBIF: http://www.gbif.org) for species of the Amazon basin (Aves, Magnoliophyta, Insecta, Squamata, Amphibia, Mammalia).

-Gathering phylogenetic clades for distributed species in the Amazon basin.

-Data cleaning under the protocol proposed by R-Alarcon., & Miranda-Esquivel. (in prep). 

-Delimitation of areas of endemism under the optimality criterion implemented in the program NDM/VNDM. 

-Localization the isolating barriers for different taxa with distribution in the Amazon basin using the Vicariance Inference Program (VIP) (Arias et al. 2011).

-Analysis of events under models (Ronquist, MC, Reconciled) implemented in TreeFitter 1.3B1 (Ronquist, 2002).

Goloboff, P. 2005. NDM/VNDM ver. 2.5. Programs for identification of areas of 

endemism. Programs and documentation available at www.zmuc.dk/public/phylogeny/endemism

Arias JS, Szumik CA, Goloboff PA (2011) Spatial analysis of vicariance: A method for using direct geographical information in historical biogeography. Cladistics 27: 617–628. doi: 10.1111/j.1096-0031.2011.00353.

Ronquist, F. 2002. TREEFITTER, version 1.3b. Computer program and manual available by anonymous FTP from Uppsala University.